ICSI technique involves the use of specialised micromanipulation tools and equipment, that allows embryologists to identify the normal-appearing, active sperm with good shape. The selected sperm is then injected into the cytoplasm of the egg. It is used in more than half of all IVF treatments and offers the most successful type of treatment for men who are having low sperm count , motility or morphology.

A man may have physiological or anatomical condition that prevents the passage of sperm in the ejaculate. In this condition the sperms can be retrieved surgically from the epididymis or the testicular tissue.

Intracytoplasmic sperm injection (ICSI) is a laboratory procedure which helps infertile couples undergoing IVF in cases of severe male factor infertility. There is now an alternative to this standard ICSI procedure known as the PICSI procedure. Through PICSI an embryologist can determine sperm selection in the same manner as it happens inside the human body.

It is defined as the amount of damage seen in the sperm DNA. i.e. the amount of abnormal genetic material present within the sperm cell, which in turn may be a reason for male subfertility, IVF failure and miscarriage. One must consider the impact of DNA damage on the sperm because half of the DNA in the offspring comes from the father’s side.

IVF with donor sperm is an option used when the male partner is medically unable to produce sperm, or has suboptimal semen parameters, or when there is a genetic problem that could be inherited from the male.