Motherhood is not easy and comes with many responsibilities. It needs your time, your energy, and you may have to compromise with your full-fledged career. Women nowadays are delaying motherhood either to focus on their job, or they might not be ready to start a family anytime soon. Some women wait for the right partner to get married. In such cases, Vitrification Technique may come as a blessing for these women who want to focus on their career at present and plan their future accordingly. Also, women undergoing cancer treatment or who experience certain autoimmune disorders may also benefit from this technique.
Vitrification process is an amazing breakthrough for preserving oocytes (egg) and embryos, and this process has transformed today’s ART industry. It is an advanced technique that leads to a glass-like formation. The word Vitrification meaning comes from the Latin word ‘Vitrum’ that means glass. In this case, the process of freezing happens so rapidly that the water molecules don’t get the time to form ice crystals, but instead immediately solidify into a glass-like structure thus avoiding freezing injuries to the eggs or embryos.
Vitrification of embryos has now become a potential alternative to slow freezing, and the process is explained in the following steps:
Vitrification is a complicated procedure than slow freezing, but this technique preserves and keeps the eggs completely fresh even after a few years. It has also been seen that pregnancy success rates from vitrification can be compared to fresh IVF cycles.
The underlying problem with freezing eggs, versus sperm or embryos, is that eggs are mostly made of water. Because of this, ice crystals would form when freezing was attempted, and these crystals would damage the cell’s structure, making them unusable. Even though doctors try to remove the crystals as they thaw the egg, but the pregnancy outcomes of these eggs were found to be very poor.
The key to the whole process is a smooth transfer of the natural fluid out of the egg, while it is made sure that the anti-freeze is slowly flowing in at the same time. Once this step is carefully done, the eggs quickly freeze or vitrify. This quick cooling keeps the ice crystals from forming that could have damaged the delicate cellular parts of the eggs. Nowadays vitrification of oocytes has become a standard part of infertility treatment.
Vitrification of embryos is done to preserve the excess embryos. In IVF, the practice is to transfer a smaller number of fresh embryos in the uterus to minimise the risk of multiple pregnancies. If the first cycle fails then without undergoing the hassles of ovarian stimulation again followed by egg retrieval, a frozen embryo transfer can be done.
Vitrification plays a significant role in the medium to severe cases of ovarian hyperstimulation syndrome (OHSS). In approximately 5-10% of cases ovarian hyperstimulation, i.e. excessive reaction of the ovaries to hormonal stimulation, occurs in women undergoing extracorporeal (occurring outside the body) fertilisation. In such situations, it is not advisable to transfer fresh embryos due to medical reasons. Therefore, all embryos are frozen and transferred after hyperstimulation has entirely subsided. In cases of polyps and hydrosalpinx, a doctor recommends frozen embryo transfer (FET).
The main advantages of vitrification over slow freezing are:
|1) Vitrification is a modern technique of cryopreservation that involves sudden cooling of reproductive cells to -196℃ within seconds.
|1) It is a technique of slow freezing of reproductive cells in a special medium and then storing them in liquid Nitrogen at -196℃
|2) In vitrification a medium containing a high dose of cryoprotectants is used, which protects the cells from damage.
|2) In slow freezing ice crystals are formed which can adversely affect the quality of frozen oocytes and embryo and hence reduces the chance of pregnancy.
At Medicover Fertility we have a highly equipped lab with state-of-the-art equipment which consists of high-end incubators for culturing of oocytes and embryos and vitrifies for cryopreservation.
Vitrification in cryopreservation is a very intricate procedure; hence is a delicate art to master. Our Embryologists are internationally trained and follow the best global practices with the latest technology. At Medicover we have been using vitrification in freezing both the oocyte and embryos. Moreover, the embryologists are available 24x7 to monitor the embryos/oocytes. Medicover Fertility has successfully conducted numerous vitrified embryo transfers with a high pregnancy outcome.